菜单
　　

    摘要：本次实验的主要目的是为了探究辛伐他汀（1×10-7 mol/L）对成骨细胞早期粘附与分化的影响。本研究首先选取SD大鼠的乳鼠（出生1-3天内）的头盖骨组织进行体外培养，待细胞爬出长满传至第三代时，取其进行分组实验，实验组给予含辛伐他汀（1×10-7 mol/L）的完全培养液对其干预，而对照组给予含等量溶剂无水乙醇的完全培养液进行干预，待培养24小时后观察其细胞的形态，分别培养4和7天后对不同基底细胞的碱性磷酸酶活性定量测定。本研究对粘附性的影响主要通过观察纽蛋白和肌动蛋白表达的情况，而对成骨分化的影响主要通过测定与底物对硝基苯酚（PNP）的反应情况来反映碱性磷酸酶酶活性大小，结果表明辛伐他汀（1×10-7 mol/L）对成骨细胞早期粘附无明显作用而对成骨细胞的形态和分化有明显的促进作用。69049

    毕业论文关键词：辛伐他汀、成骨细胞、粘附、分化、碱性磷酸酶活性

    Effect of simvastatin on early adhesion and differentiation of osteoblasts

    Abstract: The main purpose of this study was to explore the effect of simvastatin(1 x 10-7 mol / L) on the early adhesion and differentiation of osteoblasts. This study firstly isolated the skull bone tissue of newborn SD rats (postnatal 1-3 days) and cultured in vitro, the cells were passaged to the third generation and then above respective experiments were performed, including the experimental group give containing simvastatin (1 x 10-7 mol / L) in complete culture medium and the contrast group containing the same amount of solvent ethanol in the complete culture medium. when cells were cultured for 24 hours, the morphological observation was conducted, when they were cultured for 4 and 7 days, the alkaline phosphatase activities were quantitatively determined. In this study, the expression of vinculin and actin were marked as the adhesion, and the measurement of substrate p-nitrophenol (PNP) response was used to reflect the activity of the alkaline phosphatase enzyme. The results showed that simvastatin（1×10-7 mol/L）had no obvious effect on early adhesion of bone cells, bone cell morphology and differentiation were promoted by simvastatin .

    key word：Simvastatin, osteoblast, adhesion, differentiation, alkaline phosphatase activity

    目录

    引言 1

    1 材料与方法 2

    1.1供试材料 2

    1.1.1动物 2

    1.2 药品与试剂 2

    1.3 方法 2

            1.3.1DMEM高糖培养基的配置 2      

    1.3.2原代培养 2

            1.3.3成骨细胞ALP染色的鉴定 3

    1.3.4实验分组 3

    1.3.5细胞形态观察 3

            1.3.6细胞内蛋白总量测定 4

    1.3.7碱性磷酸酶活性检测 5

    2 结果与分析 5

        2.1 成骨细胞的形态及鉴定 5

    2.2 辛伐他汀对成骨细胞早期粘附的影响 6

    2.3 辛伐他汀对成骨细胞分化的影响 7

    3 讨论 8

    4 致谢 9

    参考文献 10

  1. 上一篇：中药有效成分与血浆蛋白结合的分析研究
  2. 下一篇：药用植物大戟细胞色素P450还原酶基因的克隆与功能分析

  1. OpenGL+Brunnstrom虚拟环境下对...

  2. 狼毒大戟甘草药对配伍挥发成分的GC-MS分析

  3. 小鼠肝损伤后对甘草酸药物动力学的影响研究

  4. 紫苏醛对抗甘薯黑斑病菌活性研究

  5. 铝中毒对小鼠学习记忆的影响研究

  6. 铅中毒对小鼠神经行为的影响

  7. 消极情绪对内隐攻击性的影响

  8. 杂拟谷盗体内共生菌沃尔...

  9. 当代大学生慈善意识研究+文献综述

  10. 乳业同业并购式全产业链...

  11. 大众媒体对公共政策制定的影响

  12. 电站锅炉暖风器设计任务书

  13. 十二层带中心支撑钢结构...

  14. 河岸冲刷和泥沙淤积的监测国内外研究现状

  15. 酸性水汽提装置总汽提塔设计+CAD图纸

  16. java+mysql车辆管理系统的设计+源代码

  17. 中考体育项目与体育教学合理结合的研究

　　

About

751论文网手机版...

主页：http://www.751com.cn

关闭返回