摘要细菌在矿质表面的吸附是生物浸矿的关键环节。以往对细菌在矿物表面的吸附研究多考虑的是PH值，温度，培养时间等外部因素，而本文从生物冶金工艺循环特征出发，探究夹带和溶解于萃余液中的萃取有机物对细菌吸附行为的影响。结果表明：纯培养基中A.t菌在矿石表面吸附率为55.6%，与之相比，随着萃余液中夹带相Lix984N的体积浓度从2.5%、5%、10%、15%增加，吸附率也从56.2%、58.4%、58.9%、60.7%依次增加，壬基酚体积浓度为0.003%时吸附率为59.1%也在增加。这是因为夹带有机相中Lix984N和壬基酚虽然都是对细菌有毒害作用的表面活性剂，会抑制A.t的生长与增殖，但对细菌在黄铜矿表面的吸附具有促进作用。细菌在矿石表面吸附的最佳pH为2.0，此时吸附率为58.4%。A.t菌在黄铜矿浸出过程中，浸出液中的pH在第四天是有峰值；Fe3+的量在第五天时达到最小，而Fe2+的量达到最大，这与整个浸矿过程所涉及到的离子间的交换和络合物的生成有很大的关系。20179
关键词：生物浸矿，吸附，萃余液，表面活性剂，夹带
Abstract
Bacteria adsorbed on the mineral surface is an important link in bioleaching. In the past, study on adsorption of bacteria on the mineral surface are considerated the pH, temperature,  time and other external factors. This study embarks from the circulation characteristics ofbiological metallurgy process, to study effect of extraction of organic matter of entrainment and dissolved in theraffinate of bacterial adsorption behavior. The results show that: Pure culture of A.t bacteria medium adsorption on the surface of ore rate is 55.6%,however, With the volume concentration of entrained in the raffinate phase Lix984N increased from 2.5%, 5%, 10%, 15%, 56.2%, the adsorption rate from 58.4%, 58.9%, 60.7% in turn increase,.Nonylphenol concentration at 0.003% when the adsorption rate of 59.1% is on the increase. This is because the entrained organic phase Lix984N and nonylphenol are surface active agent is toxic to bacteria, growth and proliferation could inhibit A.t, but bacteria can promote the adsorption on the surface of chalcopyrite. Bacteria in the best pH mineral surface adsorption is 2.0, the adsorption rate was 58.4%. A.t bacteria in chalcopyrite leaching process, the leaching solution of the pH is a peak on the fourth day; the amount of Fe3+ reached the minimum at fifth days, while the amount of Fe2+ reached the maximum, it has much to do with the ions in the leaching process involved in the exchange and the formation of complexes.
Keywords: Bioleaching, adsorption, raffinate, Surfactant, entrainment
 目录
1.    绪论    1
1.1    生物冶金技术简介    1
1.2    浸矿微生物的种类及其生长环境    2
1.3    微生物在矿物表面吸附的研究    3
1.4  溶剂萃取有机相夹带的研究    4
1.4.1    影响微生物在矿物表面吸附的因素    4
1.4.2    细菌在矿物质表面吸附机理的研究    5
1.4.3    浸矿细菌的氧化途径及浸出机理    5
1.5    研究的目的、意义    6
2.    实验部分    7
2.1 实验原料    7
2.2 仪器设备    8
2.3 萃取步骤与方法    9
2.3.1    Mac培养基的制备    9
2.3.2    萃取有机相配制    10
2.3.3    萃取方法    10
2.3.4    分相    10
2.4  LIX984N不同浓度对细菌在矿石表面吸附行为影响    10 铜萃取有机物对Acidithiobacillus.thiooxidans菌吸附行为的影响:http://www.751com.cn/cailiao/lunwen_11854.html