摘要：随着纳米生物技术的不断发展，纳米四氧化三铁在机械、生物医学等领域起着重要作用。本课题采用共沉淀法合成纳米四氧化三铁，并通过一步法将四氧化三铁包裹在介孔二氧化硅中，制备出具有高磁饱和强度、超顺磁性、高比表面的磁性微球。采用扫描电镜（SEM）、透射电镜（TEM）、粒径分析等方法表征对比使用不同量氨水的磁性微球。同时，选用乳腺癌细胞MCF-7为模型细胞、人血清蛋白（HAS）为模型的蛋白，考察磁性微球对细胞的生物安全性及蛋白吸附情况。并测定微球的载药性能和药物释放行为。结果表明，在介孔二氧化硅微球中引入纳米四氧化三铁，仍具有高比表面积，高孔容，大孔隙等特点，并还具有的高磁饱和强度等优异的磁响应功能。由于其较好的生物安全性，能为本课题组后期靶向药物载体研究奠定基础。63479
毕业论文关键词：磁性/介孔纳米粒子；二氧化硅；共沉淀法；细胞毒性；蛋白吸附 
Preparation and Properties of Mesoporous SiO2 Magnetic Microspheres
Abstract：With the development of Nano-biotechnology,  the magnetic Nano-Fe3O4 plays an important role in the field of mechanical, biomedical and other fields. In this paper, co-precipitation is chosen to synthetize Fe3O4.Then the Fe3O4 is wrapped in mesoporous silica by one-step method. Magnetic microspheres with high magnetic saturation, magnetic saturation, and high specific surface were prepared. SEM, TEM, size test, BET and other tests are used to compare the magnetic microspheres made by different amounts of ammonia. At the same time, the influences of the  ammonia  amount in  the magnetic microspheres  to the  cell’s biological safety and adsorption of protein were analyzed with the MCF-7 as model cell and HAS as model protein. Then, the drug loaded and the drug release behavior were also characteristic.The results show that, with the absence of Fe3O4 nanoparticles, the mesoporous silica microspheres are spherical with high specific surface area, high pore volume and pore characteristics, as well as high saturated magnetic intensity and excellent magnetic response function. Because of its good biological safety, this material could be used in the drug delivery system in the future.
Key word：Mesoporous magnetic particles; SiO2,  co-precipitation; biological safety; Protein Adsorption 

目   录 

1.绪论  ..  1 

1.1什么是介孔材料 ..  1 

1.2介孔二氧化硅   2 

1.2.1介孔二氧化硅的合成机理  .  2 

1.2.2介孔二氧化硅制备方法  ..  2 

1.3环境响应性介孔二氧化硅载体 ..  3 

1.4 磁性介孔二氧化硅微球的研究进展    4 

1.4.1 合成方法  .  4 

1.4.2 磁性介孔二氧化硅材料的合成机理  ..  5 

1.4.3磁性介孔二氧化硅应用  ..  6 

1.5本课题研究的目的和意义 .  7 

1.6本课题研究的主要内容 ..  7 

2.实验部分    9 

2.1实验原料 ..  9 

2.2主要仪器和设备 ..  9 

2.3实验方法   10 

2.3.1 MSNs的制备  ..  10 

2.3.2 Fe3O4纳米晶的制备    10 

2.3.3溶胶-凝胶法制备 Fe3O4@MSNs  . 11 

2.3.5 MSNs及磁性响应 MSNs 微球的药物担载 .  12 

2.4测试与表征 ..  12 

2.4.1介孔二氧化硅磁性微球的基础理化测试  ..  12 

2.4.2细胞毒性测试  .  13 

2.4.3人血清蛋白（HAS）黏附测试    14  磁性介孔SiO2微球的制备及性能研究:http://www.751com.cn/cailiao/lunwen_69971.html