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摘要：本实验是从互花米草的耐盐性入手，从生理方面寻找控制互花米草入侵的机制。以崇明东滩盐沼湿地的入侵植物互花米草为实验对象，开展盐胁迫实验，并以本土植物芦苇作为对照，从种子萌发时即开始定期进行不同浓度盐胁迫，盐溶液浓度设置为0g/L、5g/L、10g/L、20g/L、30g/L、40g/L、50g/L，胁迫一个半月后取两种植物的叶片测定其叶片内的膜脂过氧化产物丙二醛（MDA）含量以及保护酶过氧化物酶（POD）和超氧化物歧化酶（SOD）的活性，从而了解两种植物在盐胁迫下的受损程度及其抗逆性。结果显示，低盐浓度对互花米草的生长有促进作用，高盐浓度对其生长有抑制作用。在相同浓度的盐胁迫条件下，互花米草无论是膜脂过氧化产物丙二醛的含量，还是保护酶过氧化物酶和超氧化物歧化酶的活性都比芦苇的高，这说明互花米草的损伤程度比芦苇的高，但同时说明互花米草的抗逆性比芦苇强，这点从两种植物的生长状况就能充分证明。21010
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毕业论文关键词： 互花米草；芦苇；盐胁迫；丙二醛；过氧化物酶；超氧化物歧化酶
Responses of the activities in the leaves of Phragmites australis and Spartina alterniflora to salinity stress.
Abstract：This experiment started with the salt tolerance of Spartina to search the invasion mechanism of Spartina from the physical aspects of Spartina. The saltmarsh wetland invasive plant Spartina as the experimental subject, the native plant Phragmites as the control, to develop the salt stress experiment, which began with different concentrations of salt stress regularly from the seeds germination, the concentration of the salt solution is set to 0g/L, 5g/L, 10g/L, 20g/L, 30g/L, 40g/L, 50g/L, a half months later, taking the leaves of the two plants to determine the content of the lipid peroxidation product malondialdehyde (MDA), and the activities of the protective enzyme peroxidase (POD) and superoxide dismutase (SOD), to find out the extent of damage and resistance of the two plants under the salt stress. The results showed that low salt concentration can promote the growth of Spartina; high salt concentration has the inhibition of the growth of Spartina. At the same concentration of salt stress, Spartina either the content of lipid peroxidation product malondialdehyde, or the activates of the protective enzyme peroxidase and superoxide dismutase, are all higher than the Phragmites, indicating that the extent of the damage of Spartina is higher than Phragmites, but also describes that the resistance of Spartina is stronger than Phragmites, and it is fully proved by the growth status of the two plants.
Key words：Spartina alterniflora； Phragmites australis；salinity stress；malondialdehyde；peroxidase；superoxide dimities
目 录
1 绪论 1
1.1 研究区域概况 1
1.2 互花米草在全球范围内的概况 2
1.3 盐碱土、互花米草、芦苇、丙二醛、过氧化物酶和超氧化物歧化酶的简介 2
1.3.1 盐碱土的简介 2
1.3.2 互花米草的简介 3
1.3.3 芦苇的简介 3
1.3.4 丙二醛的简介 3
1.3.5 过氧化物酶的简介 3
1.3.6 超氧化物歧化酶的简介 4
1.4 盐胁迫对植物的影响 4
1.5 互花米草的研究前提 5
1.5.1 分布现状 5
1.5.2 研究现状 5
1.5.3 研究意义 6
1.5.4 选题依据 6
2 互花米草和芦苇的研究内容 7
2.1 实验材料和方法 7
2.1.1 研究材料 7