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摘要：苦瓜富含蛋白质、糖、文生素及各种必需氨基酸等。苦瓜不仅有良好食用价值，而且还有明显的药用功能，素有“药用蔬菜”之称。本文通过纤文素酶协同超声波法提取苦瓜中苦瓜粗多糖，分析提取条件以及优化工艺参数，最后利用提取出的苦瓜粗多糖进行抑菌性研究，为苦瓜资源在食品抑菌领域开发利用提供理论依据。本课题通过单因素实验和正交实验确定了纤文素酶协同超声波法的最佳工艺条件为：超声波功率360W、纤文素酶3500U/ml、提取温度60℃、pH5.5、料液比1:40。在此工艺条件下苦瓜多糖的提取率达到15.8%。同时使用提取的苦瓜多糖进行抑菌性研究，证明苦瓜多糖对细菌有良好的抑制作用，特别是对金黄色葡萄球菌、大肠杆菌的抑制作用显著21275
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毕业论文关键词: 苦瓜多糖；超声波；纤文素酶；抑菌性
Extraction,antimicrobial activities of Momordica Charantia polysaccharide
Abstract:Momordica charantia is rich in protein，sugar，vitamins and all kinds of essential aminoacids，etc．，and is called‘'medicinal vegetables”．Momordica Charantia polysaccharide were extracted by ultrasonic assisted extraction，Finally,the main antibacterial components were separated and the correlated bactriostasis were studied in this paper． In this study, this novel extraction method was used to isolate polysaccharides from Momordica Charantia polysaccharide, and orthogonal experiment was employed to optimize the extraction conditions. The optimal conditions for the extraction of polysaccharides were determined to be the ratio of liquid to solid of 40, pH 5.5, Ultrasonic power 360W, cellulose 3500U/ml and extraction temperature of 60℃. Under these optimal conditions, the yield of polysaccharides obtained was 15.8%. In addition, the polysaccharides showed moderate antimicrobial activity of Staphylococcus aureus ,escherichia coli. Key words: Momordica Charantia polysaccharide; Ultrasonic ; cellulose ; antimicrobial
目录
1.引言 1
1.1苦瓜生物学特性 1
1.2苦瓜多糖的作用 1
1.2.1降血糖与降血脂的作用 1
1.2.2抗病毒的作用 1
1.2.3对免疫活性的作用 2
1.2.4抑菌作用 2
1.3苦瓜有效成分的提取技术研究进展 2
1.3.1有机溶剂提取技术 2
1.3.2微波辅助提取技术 2
1.3.3超声波辅助提取 3
1.3.4超临界流体萃取技术 3
1.4研究的目的和意义 4
2.材料与方法 5
2.1.实验材料 5
2.1.1实验原料 5
2.1.2供试菌种 5
2.1.3培养基 5
2.1.4主要试剂 5
2.2试验仪器 6
2.3实验方法. 6
2.3.1苦瓜多糖的提取工艺流程 6
2.3.2葡萄糖标准曲线的制作 7
2.3.3苦瓜多糖提取的单因素实验 7
2.3.3.1超声波功率 7
2.3.3.2料液比 7
2.3.3.3.pH值 8
2.3.3.4提取温度 8
2.3.3.5纤文素酶量 9
2.3.4正交试验 9