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摘要：本实验选用从仔猪肠道筛选获得的三株乳酸菌（L1、L2、L3）及实验室保存的芽孢杆菌（B1、B2、B3）和酵母菌（J1、J2），按照体积为3:5:1的比例组合制备复合菌，将其作为接种物（接种量8%）对以玉米与豆粕（3:1）为原料配制成的的混合料发酵，并比较了不同菌株组合对发酵后饲料中的乳酸菌数量、饲料pH和乳酸含量、常规养分以及挥发性脂肪酸浓度的影响。结果显示，发酵48h后，组合为L3、B2与J1进行组合的第五组和L3、B3与J2的第751组合发酵饲料中的乳酸菌数量显著高于其他组（P<0.05）；第五组合的pH显著低于其他组（P<0.05）；乳酸含量显著高于其他组（P<0.05）；发酵后各组饲料的常规养分测定结果差异不显著（P>0.05），复合菌发酵对第五组合粗蛋白和粗脂肪有显著影响（P<0.05)；总挥发性脂肪酸含量第五组和第751组显著高于其他组合（P<0.05）。结果说明，当前条件下的最佳发酵配伍为L3、B2与J1的菌株组合。30509
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毕业论文关键词：复合益生菌；发酵；最佳配伍；筛选
Evaluation of the probiotics used to ferment feed
Abstract：In this study,three strain of lactobacillus（L1,L2,L3) screened from the gut of piglets and bacillus（B1,B2,B3）,accharomycetes（J1,J2）preserved in our lab were used to make compound probotics in the proportion 3:5:1 of volume.We used the compound probiotics to ferment feed,the feed was made up of corn and soybean at a rate of 3:1.The counts of lactic acid bacteria,pH,concentration of lactic acid,nutrient and volatile fatty acid were compared and analyzed.Group 5 was combined with L3,B2 and J1;group 6 was combined with L3,B3 and J2.After fermented for 48h,the results show that the counts of lactic acid bacteria in group 5 and group 6 were significantly higher than other groups (P<0.05).In group 5, the pH was lower than other groups significantly (P<0.05),but the concentration of lactic acid was significantly higher than other groups (P<0.05),After fermentation the nutrient comparisons were not significant ,but there are significant influence on crude protein and crude fat (P<0.05).the total volatile fatty acid in group 5 and group 6 were significantly higher than others (P<0.05).Results show that,at present environment the best fermented combination was L3,B2 and J1.
Key words:compound probiotics ; fermentation; best-combination; screen
摘要1
Abstract1
Key words1
引言2
1 材料与方法2
1.1 仪器与试剂2
1.1.1 仪器2
1.1.2 实验试剂2
1.2 材料的制备3
1.2.1培养基的制备3
1.2.2三种菌的复壮活化3
1.2.3发酵饲料的制备3
1.3试验方法3
1.3.1 试验3
1.3.2 发酵后饲料pH和乳酸含量的测定3
1.3.3 发酵饲料中粗纤文的测定4
1.3.4 发酵饲料粗脂肪的测定4
1.3.5 发酵饲料中粗蛋白的测定4
1.3.6 发酵饲料中挥发性脂肪酸的测定4
2 结果与分析 4
2.1 不同组合的复合益生菌发酵对饲料的干物质损失率和干物质的影响 4
2.2 不同组合的复合益生菌发酵饲料48h乳酸菌计数结果比较 4
2.3不同组合的复合益生菌发酵饲料对饲料pH和乳酸含量的比较5
2.4 不同组合的复合益生菌发酵饲料的常规养分的比较 5
2.5 不同组合的复合益生菌发酵饲料的挥发性脂肪酸测定的比较 6
3 讨论 6
3.1 复合益生菌发酵饲料对饲料干物质和干物质损失率的影响 6
3.2 复合益生菌发酵饲料对乳酸菌落数的影响 6
3.3复合益生菌发酵对饲料pH和乳酸含量影响7
3.4复合益生菌发酵对饲料的常规养分的影响7
3.5复合益生菌发酵对饲料中挥发性脂肪酸的影响7