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摘要：秦川牛是我国黄牛中最优秀的黄牛品种之一，我国秦川牛具有很多优秀的性状，如早熟、大理石纹明显和肉质鲜嫩等。但是，仍还有很多不足。在肉用牛饲养中，肌肉的质量和生长速度是衡量家禽生产性能的最重要指标，研究影响秦川牛肌肉生长的因素极为重要。本实验，为了探究了miRn-17基因在秦川牛不同生长时期，不同组织中的表达量差异情况，我们分别采集了胎牛，犊牛和成牛不同器官和不同肌肉作为试样，通过实验和实时荧光PCR定量并经过一系列数据处理，我们得知miRn-17在胎牛肌肉组织中的表达相对于在同期在脾、肺中的表达差异为极显著，与在肝、肾、背肌中的表达差异不显著。而在犊牛中，miRn-17在肌肉中的表达相对于肾脏、脾脏中的表达差异为极显著，与心脏、肝脏、脂肪中表达差异不显著，在肺中的表达差异显著。成年牛中，miRn-17在肌肉中表达明显突出，相对与别的组织中的表达来说差异极为显著。在不同时期秦川牛的肌肉中，miRn-17的表达量检测结果发现，在胎牛时期肌肉miRn-17表达量最高，犊牛和成牛中表达量较少且两时期表达相差不大。总体上看miRn-17在不同时期不同组织中的表达是具有较好的时间和空间表达差异性。37898
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毕业论文关键词：microRNA；病理调控；基因调控；肌肉生长
MiRn - 17 in space-time expression of qinchuan cattle groups at different time points
Abstract: Qinchuan cattle is one of the best in breed cattle, Qinchuan country has many excellent traits such as early maturity, marbling clear and the meat is tender and so on. However, there are still many shortcomings. In beef cattle rearing, muscle mass and growth is the most important measure of the performance of poultry production, research Qinchuan muscle growth factors are extremely important. In this study, in order to explore the miRn-17 gene in Qinchuan different growth stages, the expression of differences in the organization, we were collected from the fetal cattle, calves and cattle into different organs and different muscle as a sample experiments and quantitative real-time PCR and subjected to a series of data processing, we learned miRn-17 expression in fetal bovine muscle tissue relative to the same period in the spleen, lungs differential expression is very significant, and in the liver, kidney, back muscles The expression was not significantly different. In the calf, miRn-17 expression in muscle relative expression differences kidney, spleen is extremely significant, and heart, liver, fat no significant difference in the expression, the expression of differences in the lung significantly. Adult cattle, miRn-17 expression was prominent in the muscle, as opposed to other tissues is extremely significant difference. Qinchuan muscle at different times, the expression of test results miRn-17 is found in fetal calf muscle miRn-17 times the highest expression, calves and adult cattle expression and the expression of two relatively small amount of time or less. Overall miRn-17 expression in different tissues in different periods is having a good time and spatial expression differences.
Key words: MicroRNA; Pathological regulation; Gene regulation; Muscle growth
目录
引言 1
1实验材料及其试剂 2
1.1实验样本的采集 2
1.2 实验试剂 3
1.2.1 普通试剂 3
1.2.2 试剂盒类 3
1.3实验仪器 3
2实验方法步骤 4
2.1 引物设计 4
2.2 提取组织中的总RNA [9] 4
2.3 RNA的质量检测 5
2.4 RT-PCR 合成cDNA 5
2.4.1 除去总RNA中可能夹杂的基因组DNA 5
2.4.2 反转录反应 6
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