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摘要利用静电纺丝技术可以将许多粘合性材料在电场的作用下转化为小分子的纳米级材料，并在电极上富集，并在后续的实验中，利用其在电极上的螯合作用，可以与各种功能基团、分子进行结合，在分子以及纳米程度上进行各方面的电化学实验。PET溶液是一种可以在壳聚糖溶液的包裹作用下，在存在外加电场的作用时实现静电抽丝的溶液。当PET溶液利用静电作用吸附于电极表面后，纳米材料螯合作用可以使电化学实验中，原电位还原成为可能。在电化学稳定还原电位中，氯金酸溶液可以实现分解，并使金在电极表面的富集，进而使纳米金材料参与后续实验。四巯基苯硼酸溶液便是可以实现与纳米金离子进行吸附作用的溶液，而苯硼酸基团上的硼-羟基，又可以与各类具有酸性催化效应的酶、蛋白质上的羟基进行结合，从而实现对敏感酶类的保护作用、特定生物催化传感器的制备作用，本实验设计报告中，多酚氧化酶、过氧化氢酶均可实现与该种硼-羟基结合。此类静电纺丝基团引入方案，可以保护敏感而易被氧化的含有酚的酶类，也可以与正常的酸性作用酶（等电点<7.0）发生结合，从而实现进一步的蛋白质定向提取。20307
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关键词 静电纺丝 PET溶液 纳米金材料 四巯基苯硼酸 过氧化氢酶
毕业设计说明书（论文）外文摘要
Title Preparation of electrospunnano-bio-fiber performance Analysis and Application
Abstract
Many electrostatic spinning technique can be converted to the adhesive material in the electric field of Nan scale materials are small molecules , and concentrated on the electrode , and in the subsequent experiment, the effect on the chelating of the electrode , with a variety of functional groups , binding molecules , various electrochemical experiments performed in the molecule and neon extent . PET solution is a possible role in the parcel chatoyant solution , electrostatic spinning solution is achieved when the applied electric field exists. When using PET was electrostatic ally adsorbed on the electrode surface , nonmaterial’s can chelating electrochemical experiments , the reduction potential of the former is possible . In the electrochemical reduction potential of stable chlorine acid solution can be achieved decomposition, and to the electrode surface enrichment of gold , thus making gold Nan particle materials involved in follow-up experiments . Tetramercaptobenzene acid solution is possible to achieve the adsorption of gold Nan particles ion solution , phenyl bionic acid group and boron on - hydroxyls enzyme , and may have various types of acid-catalyzed reactions and the hydroxyl of the protein were combined , thereby achieving protection of sensitive enzymes , the role of specific biocatalyst preparation of the sensor, the experimental design report, polyphone oxidize, catalane can be achieved with this kind of boron - combined with hydroxyl. Such groups are introduced into the electrostatic spinning fibroin solution can protect sensitive and susceptible to oxidation enzymes containing phenols can also be the normal acidic enzymes ( bioelectric point < 7.0 ) occurs binding proteins directed to achieve a further extraction.
Keywords Electro spinning 、 PET material 、 gold Nan particle Mercaptophenylboronic acid
目 录
1 绪论 1
1.1 引言 1
1.2 静电纺丝技术简介 1
1.3 基于静电纺丝技术的纳米材料简介 2
1.4 纳米金材料简介 3
1.5 静电纺丝溶液的制备 4
1.6 静电纺丝的性能分析与条件探究 5
1.7 基于静电纺丝技术生物传感器的表征与制备 6