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摘要LED灯具有节能，光谱可调，寿命长等优点，因此目前被广泛应用于各种领域。近些年，植物组织培养也开始使用LED作为补光光源。本文以番茄为实验材料，主要通过LED灯与荧光灯的比较，来论证LED灯对番茄组织培养的影响程度。实验结果表明LED光照能够更好地促进番茄愈伤组织的发育和芽的分化。在LED灯的照射下，芽的分化速度几乎为荧光灯光照组的两倍。此外，在LED光照组中，番茄外植体能够在28天内全部完成愈伤组织的发育，而荧光灯光照组需要将近40天的时间。通过不同RB配比的LED光照实验，发现改变LED光质的配比，对番茄愈伤组织的产生有较大的促进作用，但对芽的分化速度影响不是很大。另外，通过实验可以发现当R:B=60：30时，LED灯光照既有利于番茄愈伤组织的发育又有利于芽的分化。24229
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毕业论文关键字：番茄；组织培养；LED灯；荧光灯。
The comparison of LED light and fluorescent light in Tomato tissue culture
Abstract：LED lamps with energy-saving, spectral adjustable, long life and other advantages are widely used in various fields. In recent years, LED lamps are also starting to use as the light source for plant tissue culture. In this paper, tomato leaf and stem explants were used as experiment material. The comparison of LED lamp and fluorescent lamp was performed to demonstrate the effect of LED light on tomato tissue culture. The experimental results showed that the LED light can promote the growth of tomato callus and bud differentiation. Under the irradiation of LED light, the rate of bud differentiation was almost twice as that of the fluorescent lamp irradiation group. In addition, in the LED light group, all tomato explants can induce callus within 28 days, but under the fluorescent lights it need nearly 40 days. Different ratio of RB in LED illumination has a great effect on tomato callus induction, but that on tomato bud differentiation was not observed. In addition, the experiment here also indicated that the LED light is not only beneficial to the growth of tomato callus but also to the bud differentiation when R: B = 60:30.
Keywords：Tomato; Tissue culture; LED lamp; Fluorescent lamp.
目录
1.绪论 1
1.1 植物背景介绍 1
1.1.1 番茄的物种简介 1
1.1.2 番茄的形态特征 1
1.1.3 番茄的生长习性 2
1.1.4 番茄的组织培养研究现状 3
1.1.5 白果强丰介绍（实验材料） 3
1.2 光照介绍 4
1.2.1 荧光灯介绍 4
1.2.2 LED灯介绍 5
1.2.3 LED在植物组织培养方面的应用 5
1.3 课题研究内容与意义 6
1.3.1 课题研究内容 6
1.3.2 课题研究意义 6
2 实验材料与方法 7
2.1 实验材料 7
2.1.1 实验器材 7
2.1.2 实验药品及番茄品种 7
2.2试验方法 7
2.2.1光照组设置 7
2.2.2 培养基的制作 7
2.3实验步骤 8
2.3.1 种子消毒及发芽 8
2.3.2接种 8
3 结果与分析 9
3.1 LED光照组与荧光灯光照组番茄组织培养情况 9
3.2 不同RB配比的LED光照对番茄组织培养的影响 12
4.讨论 18
致谢 18
参考文献 19
1.绪论
1.1 植物背景介绍
1.1.1 番茄的物种简介
番茄最初在秘鲁发现，大约16世纪初传入欧洲。开始人们认为番茄有毒，所以只把它作为观赏性植物栽培，直到18世纪初才开始作为蔬菜被人们接受。17～18世纪由欧洲传入亚洲。番茄约在20世纪50年代才作为蔬菜和水果开始在中国大量种植。