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摘要：本研究旨在研究长期日粮添加纳米ZnO（3-35周）对小鼠发育、锌代谢、微量元素（Zn,Fe,Cu,Mn）分布的影响。测定纳米微粒的基本特征后，将纳米ZnO添加至基础日粮中，添加水平分别为0、50、500和5000mg / kg。研究结果表明，长期日粮添加50和500 mg / kg纳米ZnO对小鼠无明显毒性作用，但日粮添加5000mg / kg纳米ZnO显著降低了体重（从第4至16周），增加了胰、脑、肺相对重量。日粮添加5000 mg / kg纳米ZnO显著提高了血清谷丙转氨酶活性和锌水平，并显著提高了空肠锌代谢相关基因的mRNA表达水平，包括MT1，MT2，ZIP8，ZIP14，ZnT1，ZnT2和ZnT4。生物分布分析结果表明，日粮添加5000mg / kg纳米ZnO显著增加肝，胰，肾和骨骼（胫骨和腓骨）中锌的含量，对组织中Cu、Fe和 Mn的含量无显著影响，但肝脏Fe含量和胰腺Mn水平除外。本研究结果提示，长期日粮添加50和500 mg / kg纳米ZnO对小鼠无明显毒性作用，但高剂量纳米ZnO（5000mg / kg）对小鼠发育有一定毒性作用，并改变了锌的生物学分布与代谢。28138
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毕业论文关键词：纳米氧化锌；小鼠；锌代谢；微量元素
Effects of Long-Term Exposure to Zinc Oxide Nanoparticles on Development, Zinc Metabolism and Biodistribution of Minerals (Zn, Fe, Cu, Mn) in Mice
Abstract: This study aimed at investigating long term effects of zinc oxide nanoparticles (nano-ZnOs) on development, zinc metabolism and biodistri-bution of minerals (Zn, Fe, Cu, and Mn) in mice from week 3 to 35. After the characteristics of nano-ZnOs were determined, they were added into the basal diet at 0, 50, 500 and 5000 mg/kg. Results indicated that added 50 and 500 mg/kg nano-ZnOs showed minimal toxicity. However, 5000 mg/kg nano-ZnOs significantly decreased body weight (from week 4 to 16) and increased the relative weights of the pancreas, brain and lung. Added 5000 mg/kg nano-ZnOs significantly increased the serum glutamic-pyruvic transaminase activity and zinc con-tent, and significantly enhanced mRNA expression of zinc metabolism-related genes, including MT 1(32.66 folds), MT 2 (31.42 folds), ZIP8 (2.21folds), ZIP14 (2.45 folds), ZnT1 (4.76 folds), ZnT2 (6.19 folds) and ZnT4 (1.82 folds). The biodistribution determination showed that there was a significant accumulation of zinc in the liver, pancreas, kidney, and bones (tibia and fibula) after receiving 5000 mg/kg nano-ZnO diet, while no signif-icant effects on Cu, Fe, and Mn levels, except for liver Fe content and pancreas Mn level. Our results demonstrated that long term exposure to 50 and 500 mg/kg nano-ZnO diets showed minimal toxicity. However, high dose of nano-ZnOs (5000 mg/kg) caused toxicity on development, and altered the zinc metabolism and biodistribution in mice.
Key words: Zinc oxide nanoparticles；mice；Zinc metabolism；Trace elements
目 录
摘要2
关键词2
Abstract2
Key words2
引言2
1 材料与方法3
1.1试验对象3
1.2实验设计3
1.3 试验材料3
1.3.1主要试剂4
1.3.2主要仪器4
1.4 样品采集4
1.5测定指标及方法4
1.5数据分析4
1.5.1生长性能4
1.5.2血清生化指标分析4
1.5.3微量元素的测定4
1.5.4空肠锌代谢相关mRNA表达的测定4
1.6数据统计5
2 结果与分析5
2．1 纳米ZnO的特征5
2．2 纳米ZnO对体重的影响5
2．3 纳米ZnO对器官重量的影响6
2．4 纳米ZnO对血清参数的影响6
2．5 纳米ZnO对所选组织的矿物质含量的影响7
2．6 纳米ZnO对粪便矿物质浓度的影响8
2．7 纳米ZnO对空肠中Zn代谢相关基因mRNA表达的影响8
3 讨论9
3.1纳米ZnO的特征9
3．2 纳米ZnO对体重和器官的影响9
3．3纳米ZnO对血清参数的影响10
3．4纳米ZnO对所选组织的矿物质含量的影响 10
3．5纳米ZnO对粪便矿物质浓度的影响10