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摘要:降压草是多年生宿根草本，全株肉质，分枝性强，它的叶和杆含有总黄酮类化合物，具有研究价值。本课题中是运用有机溶剂萃取法提取降压草中总黄酮，并改良实验装置和优化提取降压草中总黄酮的工艺条件，还对所得提取液进行了纯化，最后是对降压草做活性成分分析及化学鉴定。结果表明有机溶剂（乙醇）提取降压草样品总黄酮的最佳工艺条件是:乙醇浓度95%，料液比1∶40 g/ml，提取时间1h；此条件下黄酮含有率为 4.7%。通过液相色谱法分析纯化后的降压草提取物，得出提取物中的确时含有总黄酮。通过邻苯三酚法和DPPH法验证了降压草总黄酮具有显著抗氧化性。34633
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毕业论文关键词：有机溶剂提取法，总黄酮；降压草；抗氧化性
Extraction of Active Substance in Buck grasses
Abstract：Buck grass is a perennial herbaceous perennial, whole plant fleshy, strong branches, its leaves and stem contains flavonoid compounds, with research value. Using the organic solvent extraction of total flavonoids in grass buck, process conditions and improved experimental device and to optimize the extraction of licorice flavonoids, extract of total flavonoids were purified finally to buck grass active ingredient analysis and chemical identification. Results showed that extraction of organic solvent (ethanol) grass buck of total flavonoids of the sample, the optimum technological conditions are: 95% ethanol concentration, solid-liquid ratio of 1: 40 g / ml, extraction time was 1 hour; under the conditions of the flavonoids extraction rate was 4.7%. The extracts of the extracts were purified by HPLC, and the total flavonoids were contained in the extract.. The antioxidant activity of total flavonoids in the grass was determined by three phenol method and DPPH method.. The results showed that flavonoids in the extract of the antihypertensive herb had certain antioxidant activity.
KeyWords：Organic solvent extraction，total flavonids，buck grass，oxidation resistance
目录
1 绪论 1
1.1 降压草概述 1
1.2黄酮类化合物概述 2
1.2.1 黄酮类化合物的结构 2
1.2.2 黄酮类化合物的抗氧化性 3
1.2.3黄酮类化合物提取的国内外研究 4
1.3本课题的研究内容、目的及意义 5
2 材料和方法 6
2.1 实验材料与器材 6
2.1.1实验材料 6
2.1.2 主要仪器 6
2.1.3 主要试剂 6
2.2 分析方法 6
2.2.1液相色谱分析提取液 6
2.2.2 总黄酮的抗氧化实验 7
2.3 实验方法 9
2.3.1 探究影响降压草中总黄酮提取的因素 9
2.3.2 优化提取降压草总黄酮的工艺条件 9
2.3.3总黄酮提取率的计算 10
2.3.4液相色谱法分析 10
2.3.5最优条件下总黄酮的抗氧化性 11
2.3.6 活性物质的有机溶剂的提取工艺 11
3 结果与讨论 11
3.1 探究影响降压草中总黄酮提取的因素 12
3.1.1不同的提取剂 12
3.1.2降压草的不同部位 13
3.1.3降压草的干湿状况 13
3.1.4提取液的浓度 13
3.1.5不同的料液比 14
3.1.6提取时间 14
3.2 优化提取降压草总黄酮的工艺条件 15
3.2.1三水平三因素表 15