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摘要：背景：菊花多糖作为菊花的主要活性成分之一，其抗氧化活性受到各界学者的关注。目的：比较和检测两种大小金丝菊的体外抗氧化活性。方法：通过水提醇沉法提取大小两种金丝菊多糖，分别利用邻二氮菲-Fe2+氧化法、邻苯三酚自氧化法、盐酸萘乙二胺法等测定菊花多糖的清除羟基自由基(•OH)、超氧阴离子自由基（O2-•）、亚硝酸盐（NO2-）的能力以及总还原力，来比较和评价两种菊花多糖的抗氧化活性。结果：大小两种菊花多糖的得率分别是：14.86%和16.13%；大小菊花多糖对各种自由基均有一定的清除能力，但是能力不同；计算得出大菊花多糖的半数清除浓度分别为：500μg/mL、830μg/mL、58μg/mL；而小菊花多糖的半数清除浓度为1200μg/mL、125μg/mL、54.2μg/mL，两者总还原力随着浓度的增加吸光度增加，抗氧化活性增强。结论：大小两种菊花均具有一定的体外抗氧化活性，本文对两种菊花多糖的抗氧化活性进行比较和检测，对菊花的开发具有十分重要意义。51866
毕业论文关键词：金丝菊、多糖、抗氧化活性
Detection of antioxidant activity in vitro of chrysanthemum polysaccharide
Abstract: Background: Chrysanthemum polysaccharides as one of the major components of chrysanthemum, its antioxidant activity was paid attention by the scholars from all over the word. Objective: To compare and detect the antioxidant activity in vitro of two kinds of golden chrysanthemum. Methods: The polysaccharide was extracted from two kinds of golden chrysanthemum using water extract-alcohol precipitation method. To compare and evaluate the two kinds of chrysanthemum polysaccharide antioxidant activity, the scavenging activity to hydroxyl radicals(•OH), superoxide anion radical(O2-•), nitrite(NO2-) and the total reducing power were detected using the adjacent two nitrogen Fe2+ oxidation process, the development of three autoxidation method, hydrochloric acid naphthalene ethylenediamine method, respectively. Results: The yields of the two kinds of chrysanthemum polysaccharide were 14.86% and 16.13%. The two kinds of chrysanthemum polysaccharide showed certain antioxidant activity. The two kinds of chrysanthemum polysaccharide had effect on scavenging •OH, •O2- and NO2- and hydroxyl radical with the half elimination concentration of 500μg/mL, 830μg/mL, 58μg/mL and 1200μg/mL, 125μg/mL, 54.2μg/mL, respectively. The total reducing power increased with the increase of concentration. Conclusion: The two kinds of chrysanthemum polysaccharide exhibited certain antioxidant activity. This study compares and detect the antioxidant activity of polysaccharide, providing a theoretical basis for the further exploitation of chrysanthemum polysaccharide.
Key words: golden chrysanthemum; Polysaccharide; Antioxidant activity
目录
前言 1
1 材料与方法 2
1.1供试材料 2
1.1.1实验材料 2
1.1.2实验试剂 2
1.1.3仪器与设备 2
1.2 实验方法 2
1.2.1金丝菊多糖的提取 2
1.2.2 清除羟基自由基（-OH）的测定 3
1.2.3 超氧阴离子自由基（O2-）清除能力测定 3
1.2.4 亚硝酸盐（NO2-)清除能力测定 3
1.2.5 总还原力的测定 4
2 结果与分析 4
2.1 菊花多糖的提取